Primary Identifier | MGI:2387422 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Evpl |
Transmission | Germline | Strain of Origin | 129/Sv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The gene was disrupted by deleting and replacing exons 1-8 with an IRES-lacZ-neo cassette via homologous recombination. The insertion introduces stop codons in all 3 reading frames. Absence of gene expression in homozygous mutant animals was verified by RT-PCR analysis of skin samples using primers to both the 5' end and the 3' end of the gene. |