Primary Identifier | MGI:6455469 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Rock2 |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A LoxP site was inserted into intron 1 and a second loxP site and an FRT site flanked neomycin resistance gene cassette into intron 2. Exon 2 was deleted through subsequent cre-mediated deletion (Tek-cre transgene). Southern blotting, PCR, and western blotting experiments confirmed deletion of the exon and lack of expression from the allele. |