| First Author | Brookman KW | Year | 1994 |
| Journal | Genomics | Volume | 22 |
| Issue | 1 | Pages | 180-8 |
| PubMed ID | 7959765 | Mgi Jnum | J:19413 |
| Mgi Id | MGI:67583 | Doi | 10.1006/geno.1994.1359 |
| Citation | Brookman KW, et al. (1994) Isolation and characterization of mouse Xrcc-1, a DNA repair gene affecting ligation. Genomics 22(1):180-8 |
| abstractText | Human DNA repair gene XRCC1 complements the strand-break rejoining defect in Chinese hamster mutant EM9 and encodes a protein that is apparently required for optimal activity of DNA ligase III. Toward the goal of producing transgenic mice that carry a mutation in the Xrcc-1 locus, the murine homolog of XRCC1 was cloned from both cosmid genomic and cDNA libraries. Upon transfection into EM9 cells, cosmids containing the functional mouse gene efficiently corrected (94-100%) the high sister-chromatid-exchange defect. Mouse Xrcc-1 is 26 kb in length, contains 17 exons, and maps by metaphase in situ hybridization to the 7A3-7B2 region of mouse chromosome 7. Isolated cDNA clones were highly truncated and were extended by anchored polymerase chain reactions. The 1893-bp open reading frame of mouse Xrcc-1 encodes 631 amino acids, compared with 633 for the human homolog. The predicted mouse Xrcc-1 protein of 69.1 kDa and pI of 5.95 is 86% identical and 93% similar to human XRCC1. |
