Primary Identifier | MGI:6324506 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Mafk |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A portion of exon 2 and all of exon 3 were replaced by a cassette inserted downstream of the translational start codon and containing lacZ and floxed neo genes. RNAse protection assays were utilized to confirm the absence of transcript in homozygous mutant mice. Expression of beta-galactosidase was driven by the endogenous promoter. The loxP-flanked neomycin selection cassete was removed via cre-mediated recombination. |