| First Author | Sofueva S | Year | 2013 |
| Journal | EMBO J | Volume | 32 |
| Issue | 24 | Pages | 3119-29 |
| PubMed ID | 24185899 | Mgi Jnum | J:206180 |
| Mgi Id | MGI:5548043 | Doi | 10.1038/emboj.2013.237 |
| Citation | Sofueva S, et al. (2013) Cohesin-mediated interactions organize chromosomal domain architecture. EMBO J 32(24):3119-29 |
| abstractText | To ensure proper gene regulation within constrained nuclear space, chromosomes facilitate access to transcribed regions, while compactly packaging all other information. Recent studies revealed that chromosomes are organized into megabase-scale domains that demarcate active and inactive genetic elements, suggesting that compartmentalization is important for genome function. Here, we show that very specific long-range interactions are anchored by cohesin/CTCF sites, but not cohesin-only or CTCF-only sites, to form a hierarchy of chromosomal loops. These loops demarcate topological domains and form intricate internal structures within them. Post-mitotic nuclei deficient for functional cohesin exhibit global architectural changes associated with loss of cohesin/CTCF contacts and relaxation of topological domains. Transcriptional analysis shows that this cohesin-dependent perturbation of domain organization leads to widespread gene deregulation of both cohesin-bound and non-bound genes. Our data thereby support a role for cohesin in the global organization of domain structure and suggest that domains function to stabilize the transcriptional programmes within them. |
