| Primary Identifier | MGI:4360671 | Allele Type | Targeted |
| Gene | Nphs2 | Transmission | Germline |
| Strain of Origin | 129S2/SvPas | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Two nucleotide substitutions (c.505G>A, c.506A>G) were inserted into exon 3 and a floxed pgk-hygromycin resistance cassette was inserted into intron 3 via homologous recombination. The hygromycin resistance cassette was then removed via Cre mediated recombination. Northern blot and real time PCR analysis indicate increased transcription compared to wild-type controls. Western blot analysis revealed decreased protein expression. Immunoflourescence analysis of podocytes from homozygous mutants indicates mislocalization of the mutant protein. |
