| First Author | Wang Y | Year | 2021 |
| Journal | Neurobiol Dis | Volume | 147 |
| Pages | 105149 | PubMed ID | 33132163 |
| Mgi Jnum | J:305151 | Mgi Id | MGI:6503639 |
| Doi | 10.1016/j.nbd.2020.105149 | Citation | Wang Y, et al. (2021) Calpain-2 activation in mouse hippocampus plays a critical role in seizure-induced neuropathology. Neurobiol Dis 147:105149 |
| abstractText | Calpain has been proposed to play a critical role in the development of epilepsy. Here we used conditional calpain-2 knock-out (C2CKO) mice in a C57/Bl6 background and a selective calpain-2 inhibitor to analyze the role of calpain-2 in epilepsy. Neurodegeneration was evident in various hippocampal subfields, in particular in mossy cells in the hilus of the dentate gyrus (DG) in C57/Bl6 mice 7 days after kainic acid (KA)-induced seizures. Calpain-2 activation was still observed in mossy cells 7 days after seizures. Calpain activation, astroglial and microglial activation, neurodegeneration, and cognitive impairment were absent in C2CKO mice and in C57/Bl6 mice treated with a selective calpain-2 inhibitor for 7 days after seizure initiation. Levels of the potassium chloride cotransporter 2 (KCC2) were decreased in mossy cells 7 days after seizures and this decrease was prevented by calpain-2 deletion or selective inhibition. Our results indicate that prolonged calpain-2 activation plays a critical role in neuropathology following seizures. A selective calpain-2 inhibitor could represent a therapeutic treatment for seizure-induced neuropathology. |
