Primary Identifier | MGI:6471922 | Allele Type | Endonuclease-mediated |
Attribute String | Recombinase | Gene | Sox1 |
Transmission | Germline | Strain of Origin | C57BL/6NCrlCrlj |
Is Recombinase | true | Is Wild Type | false |
description | ES cells = B6NJ-22(UTR) (C57BL/6NCrlCrlj) |
molecularNote | CRISPR-targeting introduced at the end of the coding sequence in exon 1 a GSG (Gly-Ser-Gly)-linker, porcine teschovirus-1 2A (P2A) peptide, SV40 virus NLS, bacteriophage P1 cre recombinase gene, and puromycin resistance cassette flanked by FRT sites (FRT/F3/F5). Flp-mediated recombination removed the selection cassette. |