Primary Identifier | MGI:3620081 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ofd1 |
Transmission | Germline | Strain of Origin | 129S2/SvPas |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exons 4 and 5 were flanked by loxP sites via homologous recombination. The Frt flanked neo cassette was removed by Flp recombinase in the ES cells. After Cre mediated recombination RT-PCR confirmed absence of these exons from transcripts in mutants and Western blot analysis failed to detect protein from recombined embryos. This allele was generated from Ofd1tm2.1Bfra |