| First Author | Yang F | Year | 2013 |
| Journal | J Atheroscler Thromb | Volume | 20 |
| Issue | 3 | Pages | 267-76 |
| PubMed ID | 23221024 | Mgi Jnum | J:203378 |
| Mgi Id | MGI:5526909 | Doi | 10.5551/jat.14381 |
| Citation | Yang F, et al. (2013) Real-time analysis of the circadian oscillation of the Rev-Erb beta promoter. J Atheroscler Thromb 20(3):267-76 |
| abstractText | AIM: Rev-Erb beta gene plays crucial roles in circadian rhythm, lipid and glucose metabolism, and several diseases. The molecular mechanisms of the transcriptional regulation of Rev-Erb beta that generate and determine the phase of the circadian oscillation remain unclear. METHODS: We analyzed the Rev-Erb beta promoter by luciferase reporter assays, real-time bioluminescence monitoring assays and electrophoretic mobility shift assays. RESULTS: Luciferase reporter assays indicated that only the 5' region and exon 1 have obvious promoter activity. Real-time bioluminescence monitoring assays revealed that E1, E2, E3, D boxes are important for maintenance of the amplitude of Rev-Erb beta oscillation. Based on EMSA results, REV-ERBbeta binds ROREs in the Bmal1 promoter region and inhibits Bmal1 promoter activity. CONCLUSION: We provide direct evidence that three E-boxes and one D-box located in the first intron are crucial for the phase of circadian oscillation in Rev-Erb beta expression and that the sequences upstream from its transcription start site function as a promoter with no circadian regulation. We also found that the E1 box affects the Rev-Erb beta oscillation phase. Our results offer new insight into the role of Rev-Erb beta in the circadian rhythm system. |
