| Primary Identifier | MGI:7607006 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Gtf2ird1)KrnJvvw |
| Strain of Origin | C57BL/6NTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A bacterial artificial chromosome which contained the entirety of the 100 kb Gtf2ird1 gene, and 89 kb of flanking regulatory sequence (60 kb at the 5â end and 28 kb at the 3â), but no additional intact genes or their promoters was utilized to generate the transgene. This was then recombined using standard methods to insert an HA tag in-frame directly before the stop codon of the beta isoforms 208,209. Homologous recombination via transient expression of RecA was employed, followed by Neomycin selection of an inserted FRT-flanked cassette. The selection cassette was then removed after the expression of FLPe, leaving a single FRT site downstream of the stop codon. |
