| First Author | Pak MD | Year | 1991 |
| Journal | Proc Natl Acad Sci U S A | Volume | 88 |
| Issue | 10 | Pages | 4386-90 |
| PubMed ID | 2034678 | Mgi Jnum | J:11182 |
| Mgi Id | MGI:59624 | Doi | 10.1073/pnas.88.10.4386 |
| Citation | Pak MD, et al. (1991) mShal, a subfamily of A-type K+ channel cloned from mammalian brain. Proc Natl Acad Sci U S A 88(10):4386-90 |
| abstractText | We have cloned and expressed a mouse brain cDNA, mShal, that encodes a transient, A-type K+ current. mShal, the vertebrate homolog of the Drosophila Shal gene, defines a distinct subfamily of voltage-gated K+ channels. The Shal deduced proteins are more highly conserved between mouse and Drosophila than other presently known K+ channels. mShal carries a low-threshold A-type current with a hyperpolarized steady-state inactivation midpoint. Marked similarity was observed between mShal and its Drosophila homolog, fShal, with regard to voltage sensitivity of activation, macroscopic inactivation, steady-state inactivation, and 4-aminopyridine sensitivity. Sequence conservation for Shal proteins is unusually high at the amino terminus, an area considered important for inactivation. Removal of conserved amino-terminal residues from mShal modifies macroscopic inactivation but the transient nature of the current is preserved. Underlying the very high conservation of mShal and fShal may be a role in the nervous system that is conserved in widely divergent species. |
