| First Author | Fan J | Year | 2006 |
| Journal | Biochem Biophys Res Commun | Volume | 340 |
| Issue | 1 | Pages | 8-12 |
| PubMed ID | 16343427 | Mgi Jnum | J:104175 |
| Mgi Id | MGI:3611420 | Doi | 10.1016/j.bbrc.2005.11.152 |
| Citation | Fan J, et al. (2006) Identification of a novel male germ cell-specific gene TESF-1 in mice. Biochem Biophys Res Commun 340(1):8-12 |
| abstractText | Mammalian spermatogenesis is precisely regulated by many germ cell-specific factors. In search for such a germ cell-specific factor, we have identified a novel mouse gene testis-specific factor 1 (TESF-1). Messenger RNA of TESF-1 was found only in the testis and its expression appeared to be regulated in a developmental manner. Further analysis demonstrated that the expression of TESF-1 was specifically in male germ cells, supported by the observation that we were not able to detect the TESF-1 mRNA from at/at homozygous mutant testes, which lack germ cells. The deduced amino acid sequence of TESF-1 contains a leucine-zipper motif, a potential nuclear localization signal, and two cAMP- and cGMP-dependent protein kinase phosphorylation sites. The green fluorescent protein (GFP)-tagged TESF-1 fusion protein was expressed in COS-7 cells and localized primarily in the nucleus. Taken together, these results indicate that TESF-1 is a novel male germ cell-specific gene, and its protein product may function as a nuclear factor involved in the regulation of spermatogenesis. |
