Primary Identifier | MGI:6195004 | Allele Type | Targeted |
Attribute String | Conditional ready, Humanized sequence, Inserted expressed sequence | Gene | Col17a1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The mouse NC14A domain (exons 17-18) was replaced with the human NC16A counterpart encoded by exons 18 and 19, which were flanked by loxP sites. A FRT-flanked neo cassette was removed via flp-mediated recombination. RT-PCR and immunofluorescence confirmed expression of the human domain. |