Primary Identifier | MGI:5790470 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Slc25a5 |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to insert a loxP site upstream of exon 2, and an FRT-splice donor/acceptor-IRES-beta-gal/neo-FRT-loxP downstream of exon 3. ES cells were transiently transfected with a FLP-expressing plasmid to remove the SA-IRES-betageo cassette leaving exons 2-3 floxed. |