| First Author | Doxsey SJ | Year | 1994 |
| Journal | Cell | Volume | 76 |
| Issue | 4 | Pages | 639-50 |
| PubMed ID | 8124707 | Mgi Jnum | J:17138 |
| Mgi Id | MGI:65190 | Doi | 10.1016/0092-8674(94)90504-5 |
| Citation | Doxsey SJ, et al. (1994) Pericentrin, a highly conserved centrosome protein involved in microtubule organization [see comments]. Cell 76(4):639-50 |
| abstractText | Antisera from scleroderma patients that react widely with centrosomes in plants and animals were used to isolate cDNAs encoding a novel centrosomal protein. The nucleotide sequence is consistent with a 7 kb mRNA and contains an open reading frame encoding a protein with a putative large coiled-coil domain flanked by noncoiled ends. Antisera recognize a 220 kd protein and stain centrosomes and acentriolar microtubule-organizing centers, where the protein is localized to the pericentriolar material (hence, the name pericentrin). Anti-pericentrin antibodies disrupt mitotic and meiotic divisions in vivo and block microtubule aster formation in Xenopus extracts, but do not block gamma-tubulin assembly or microtubule nucleation from mature centrosomes. These results suggest that pericentrin is a conserved integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays. |
