| First Author | Kajiro M | Year | 2011 |
| Journal | PLoS One | Volume | 6 |
| Issue | 10 | Pages | e25871 |
| PubMed ID | 22028794 | Mgi Jnum | J:179766 |
| Mgi Id | MGI:5303027 | Doi | 10.1371/journal.pone.0025871 |
| Citation | Kajiro M, et al. (2011) The E3 ubiquitin ligase activity of Trip12 is essential for mouse embryogenesis. PLoS One 6(10):e25871 |
| abstractText | Protein ubiquitination is a post-translational protein modification that regulates many biological conditions. Trip12 is a HECT-type E3 ubiquitin ligase that ubiquitinates ARF and APP-BP1. However, the significance of Trip12 in vivo is largely unknown. Here we show that the ubiquitin ligase activity of Trip12 is indispensable for mouse embryogenesis. A homozygous mutation in Trip12 (Trip12(mt/mt)) that disrupts the ubiquitin ligase activity resulted in embryonic lethality in the middle stage of development. Trip12(mt/mt) embryos exhibited growth arrest and increased expression of the negative cell cycle regulator p16. In contrast, Trip12(mt/mt) ES cells were viable. They had decreased proliferation, but maintained both the undifferentiated state and the ability to differentiate. Trip12(mt/mt) ES cells had increased levels of the BAF57 protein (a component of the SWI/SNF chromatin remodeling complex) and altered gene expression patterns. These data suggest that Trip12 is involved in global gene expression and plays an important role in mouse development. |
