| First Author | Rempel SA | Year | 2007 |
| Journal | Genes Immun | Volume | 8 |
| Issue | 3 | Pages | 262-74 |
| PubMed ID | 17344888 | Mgi Jnum | J:147548 |
| Mgi Id | MGI:3841362 | Doi | 10.1038/sj.gene.6364388 |
| Citation | Rempel SA, et al. (2007) Splenic and immune alterations of the Sparc-null mouse accompany a lack of immune response. Genes Immun 8(3):262-74 |
| abstractText | Sparc-null mice have been used as models to assess tumor-host immune cell interactions. However, it is not known if they have a competent immune system. In this study, the immune systems of Sparc wild-type and null mice were compared. Mice were assessed for differences in total body weight, spleen weight and spleen-to-body weight ratios. Spleens were compared with respect to morphology, and Sparc, Ki-67, MOMA-1 and IgM expression. Immune cells in blood, bone marrow and spleen were assessed by blood smears, automated blood panel, and flow cytometry. Additionally, the ability of Sparc-null mice to respond to immune challenge was evaluated using a footpad model. The morphological and immunohistochemical results indicated that Sparc-null spleens had more white pulp, hyperproliferative B cells in the germinal centers, and decreased marginal zones. Sparc-null spleens lacked normal Sparc expression in red and white pulp, marginal zones, endothelial and sinusoidal cells. By flow analysis, B cells were decreased and T cells were increased in the bone marrow. Finally, Sparc-null mice were unable to mount an immune response following footpad lipopolysaccharide challenge. These data confirm that Sparc-null mice have an impaired immune system. |
