First Author | Suzuki R | Year | 1995 |
Journal | Gene | Volume | 152 |
Issue | 2 | Pages | 257-60 |
PubMed ID | 7835711 | Mgi Jnum | J:23226 |
Mgi Id | MGI:70995 | Doi | 10.1016/0378-1119(94)00713-3 |
Citation | Suzuki R, et al. (1995) Sequences of two porcine glutamic acid decarboxylases (65- and 67-kDa GAD). Gene 152(2):257-60 |
abstractText | The cDNAs encoding the porcine 65- and 67-kDa glutamic acid decarboxylases (GAD65 and GAD67, respectively) were cloned by the PCR method. The 2246-nucleotide (nt) GAD65 cDNA contained an open reading frame (ORF) coding for a protein of 585 amino acids (aa), and the 3262-nt GAD67 cDNA contained an ORF coding for a protein of 594 aa. A remarkable conservation was shown when the deduced aa sequences of porcine GAD65 and GAD67 were compared with those of other mammalian species (human, cat and rat). Porcine GAD65 is 96% identical to human and rat GAD65, and porcine GAD67 is more than 95% identical to human, cat and rat GAD67 at the aa level. |