| First Author | Stanton ML | Year | 2005 |
| Journal | J Immunol | Volume | 174 |
| Issue | 6 | Pages | 3164-8 |
| PubMed ID | 15749844 | Mgi Jnum | J:97689 |
| Mgi Id | MGI:3576141 | Doi | 10.4049/jimmunol.174.6.3164 |
| Citation | Stanton ML, et al. (2005) Stat5 mediates the IL-7-induced accessibility of a representative D-Distal VH gene. J Immunol 174(6):3164-8 |
| abstractText | During B cell development, discrete domains within the Igh locus are activated independently for recombination. The D-distal V(H) genes are uniquely dependent on IL-7R signaling, which is thought to establish local chromatin accessibility through an unknown mechanism. To dissect this mechanism, we used a murine B cell line that responds to IL-7 by specifically inducing accessibility of a representative D-distal gene (A1) but not a D-proximal gene (V11). We demonstrate that IL-7-activated Stat5 is recruited rapidly to the A1 gene, with a concomitant increase in germline transcription and H4 acetylation. Furthermore, retroviral transduction of dominant negative or constitutively active Stat5 demonstrated that Stat5 activation is both necessary and sufficient for the IL-7-induced A1 germline transcription. Lastly, as with all known Stat5 target genes, A1 germline transcription requires a deacetylase activity. These results demonstrate that in response to IL-7 signaling, Stat5 is recruited to the D-distal A1 gene and induces accessibility. |
