| First Author | Reina-San-Martin B | Year | 2007 |
| Journal | Eur J Immunol | Volume | 37 |
| Issue | 1 | Pages | 235-9 |
| PubMed ID | 17183606 | Mgi Jnum | J:117038 |
| Mgi Id | MGI:3695503 | Doi | 10.1002/eji.200636789 |
| Citation | Reina-San-Martin B, et al. (2007) Enhanced intra-switch region recombination during immunoglobulin class switch recombination in 53BP1(-/-) B cells. Eur J Immunol 37(1):235-9 |
| abstractText | Immunoglobulin class switch recombination (CSR) is initiated by activation-induced cytidine deaminase (AID), an enzyme that deaminates cytidine residues in single-stranded DNA. U:G mismatches created by AID are processed to produce lesions that recruit and activate DNA damage response proteins including Ataxia-telangiectasia mutated (ATM), histone H2AX, Nijmegen breakage syndrome 1 (Nbs1), and p53 binding protein 1 (53BP1). Among these proteins, absence of 53BP1 produces the most severe impairment of class switching. Here, we demonstrate that AID is targeted normally to switch region DNA and that intra-switch region recombination is enhanced in 53BP1(-/-) B cells. In addition, Smicro-Sgamma1 switch region junctions cloned from 53BP1(-/-) B cells show unusual insertions suggestive of failed class switching. Our data are consistent with a role for 53BP1 in stabilizing the synapsis of switch regions during CSR. |
