| Primary Identifier | MGI:6307062 | Allele Type | Endonuclease-mediated |
| Attribute String | Modified isoform(s) | Gene | Nfix |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology generated an in-frame 24-bp deletion in exon 7 from position +49,561 to +49,584 relative to the translation start site. This deletion causes skipping of exon 7 and alternative splicing of exon 6 to exon 8 to produce wild-type Nfix isoforms. MEFs express the mutant long isoform (293 bp) and the wild-type short isoform lacking exon 7 (194 bp). |
