| First Author | Najakshin AM | Year | 1993 |
| Journal | Mol Immunol | Volume | 30 |
| Issue | 13 | Pages | 1205-12 |
| PubMed ID | 8105375 | Mgi Jnum | J:17026 |
| Mgi Id | MGI:65081 | Doi | 10.1016/0161-5890(93)90139-3 |
| Citation | Najakshin AM, et al. (1993) cDNA clones encoding mink immunoglobulin lambda chains. Mol Immunol 30(13):1205-12 |
| abstractText | Screening of a mink cDNA library with an antibody probe resulted in the isolation of clone pIGL-2 containing an Ig lambda chain coding sequence. The sequence comprised almost the entire V segment as well as J, C, and 3'-untranslated sequences. A second clone, pIGL-10, was isolated by rescreening the cDNA library with the use of pIGL-2 as a probe. pIGL-10 was found to contain a frameshift deletion of a single nucleotide in the C region. pIGL-2 and pIGL-10 were 81% homologous to each other in the FR3 of the V segment, and 95% of homology was found in their C regions. The J segments of the two clones differed in only one nucleotide position. Comparison of cloned lambda chain sequences with those of other mammals revealed that mink V lambda and C lambda genes have the highest homology with their human counterparts. The V lambda sequence of clone pIGL-2 appears to be a homologue of human subgroup III V lambda genes. Southern blot hybridization of mink DNA with the C lambda and V lambda probes derived from pIGL-2 revealed five or six hybridizing C lambda fragments and at least 11 hybridizing V lambda fragments. This suggested that the lambda genes in carnivores, like those in primates, have duplicated extensively during evolution. |
