| First Author | Nishida H | Year | 2005 |
| Journal | BMC Genet | Volume | 6 |
| Pages | 10 | PubMed ID | 15720718 |
| Mgi Jnum | J:101828 | Mgi Id | MGI:3605554 |
| Doi | 10.1186/1471-2156-6-10 | Citation | Nishida H, et al. (2005) A novel replication-independent histone H2a gene in mouse. BMC Genet 6(1):10 |
| abstractText | BACKGROUND: An uncharacterized histone H2a-coding transcript (E130307C13) has been cloned from a mouse full-length cDNA library. This transcript is encoded on chromosome 6, approximately 4 kb upstream of a histone H4 gene, Hist4h4. The proteins encoded by this transcript and the human H2afj mRNA isoform-2 have the highest amino acid similarity. In this paper, we characterize it from the expression pattern given by quantitative RT-PCR. RESULTS: Quantitative RT-PCR indicated that the gene that encodes E130307C13 (E130307C13) is regulated in a replication-independent manner, and therefore it is H2afj. Certainly, H2afj transcript lacks a stem-loop structure at the 3'-UTR but contains a poly (A) signal. In addition, its promoter region has a different structure from those of the replication-dependent histone H2a genes. CONCLUSION: The bioinformatics imply that E130307C13 is a replication-independent H2a gene. In addition, quantitative RT-PCR analysis shows that it is replication-independent. Thus, it is H2afj, a novel replication-independent H2a gene in mouse. |
