| First Author | Feng Y | Year | 2000 |
| Journal | Neuron | Volume | 28 |
| Issue | 3 | Pages | 665-79 |
| PubMed ID | 11163258 | Mgi Jnum | J:66488 |
| Mgi Id | MGI:1928549 | Doi | 10.1016/s0896-6273(00)00145-8 |
| Citation | Feng Y, et al. (2000) LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome. Neuron 28(3):665-79 |
| abstractText | LIS1, a microtubule-associated protein, is required for neuronal migration, but the precise mechanism of LIS1 function is unknown. We identified a LIS1 interacting protein encoded by a mouse homolog of NUDE, a nuclear distribution gene in A. nidulans and a multicopy suppressor of the LIS1 homolog, NUDF. mNudE is located in the centrosome or microtubule organizing center (MTOC), and interacts with six different centrosomal proteins. Overexpression of mNudE dissociates gamma-tubulin from the centrosome and disrupts microtubule organization. Missense mutations that disrupt LIS1 function block LIS1-mNudE binding. Moreover, misexpression of the LIS1 binding domain of mNudE in Xenopus embryos disrupts the architecture and lamination of the CNS. Thus, LIS1-mNudE interactions may regulate neuronal migration through dynamic reorganization of the MTOC. |
