Primary Identifier | MGI:6460450 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Tmem95 |
Strain of Origin | (CBA x C57BL/6)F2 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/cas9 mediated recombination created a 4bp substitution (ACTT to GAAG) followed by a 10 bp deletion (CGGCTTCGTC) in exon 1 predicted to result in a truncated transcript. Immunohistochemical analysis confirmed the absence of protein expression in sperm from homozygous mice. |