| First Author | Zhao Y | Year | 1995 |
| Journal | J Biol Chem | Volume | 270 |
| Issue | 41 | Pages | 24428-34 |
| PubMed ID | 7592657 | Mgi Jnum | J:29352 |
| Mgi Id | MGI:76877 | Doi | 10.1074/jbc.270.41.24428 |
| Citation | Zhao Y, et al. (1995) Expression and function of bicarbonate/chloride exchangers in the preimplantation mouse embryo. J Biol Chem 270(41):24428-34 |
| abstractText | Bicarbonate/chloride (HCO3-/Cl-) exchangers regulate intracellular pH in the alkaline range. Previously, it has been shown that mouse embryos at the two-cell stage exhibit this activity, but that the otherwise ubiquitous mechanisms for regulating intracellular pH in the acid-to-neutral range are undetectable. We have examined mouse embryos during preimplantation development (one-cell zygote through blastocyst) to determine whether HCO3-/Cl- exchange activity exists at all stages, whether it is necessary for preimplantation development, and whether messenger RNAs from the known HCO3-/Cl- exchanger genes are expressed. We have found that all stages of preimplantation embryo have detectable HCO3-/Cl- exchange activity. In addition, inhibition of this activity with the stilbene anion exchange inhibitor DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid) disrupts intracellular pH homeostasis and markedly inhibits embryo development from the two-cell stage to blastocysts in culture under conditions of moderately high external pH. Finally, mRNA encoding two members of the band 3-related AE anion exchanger gene family are expressed in preimplantation embryos. |
