| Primary Identifier | MGI:5319536 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change, Reporter | Gene | Igs1 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The following elements were inserted, in order, 2 kb 5' of the Ubb gene by targeted recombination in 129-derived ES cells: CMV enhancer/beta-actin promoter (CAG), loxP site, beta-geo, 3 transcription sites, loxP site, mitochondria targeted GFP fused to a C-terminal V5 epitope, and a bovine growth hormone 3' UTR. Mitochondrial targeting was achieved by using the N-terminal 25 amino acid targeting signal of mouse Cox8a. The V5 epitope encodes amino acid residues 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5. |
