| First Author | Mohrs K | Year | 2005 |
| Journal | Immunity | Volume | 23 |
| Issue | 4 | Pages | 419-29 |
| PubMed ID | 16226507 | Mgi Jnum | J:113279 |
| Mgi Id | MGI:3665347 | Doi | 10.1016/j.immuni.2005.09.006 |
| Citation | Mohrs K, et al. (2005) A two-step process for cytokine production revealed by IL-4 dual-reporter mice. Immunity 23(4):419-29 |
| abstractText | To monitor IL-4 expression at the single-cell level, we generated mice with insertions of different reporter genes into both copies of the Il4 gene that permitted the simultaneous analysis of IL-4 transcripts via GFP and IL-4 protein secretion by use of huCD2. Innate and adaptive cells competent for IL-4 production were marked by GFP, while cells that presently or recently secreted IL-4 additionally displayed huCD2. After challenge with the strictly enteric helminth, Heligmosomoides polygyrus, GFP-positive innate and adaptive cells disseminated widely, but IL-4 secretion was predominantly mediated by CD4+ T cells in the intestines and draining lymphoid organs. IL-4-competent cells persisted in cured animals, and memory responses reflected rapid cytokine production at the site of rechallenge. These data reveal a two-step process for cytokine production: the first generating poised cells that disseminate systemically and the second inducing the rapid production of the cytokine in response to local stimulation. |
