| First Author | van de Graaf SF | Year | 2006 |
| Journal | Mol Cell Biol | Volume | 26 |
| Issue | 1 | Pages | 303-12 |
| PubMed ID | 16354700 | Mgi Jnum | J:104196 |
| Mgi Id | MGI:3611504 | Doi | 10.1128/MCB.26.1.303-312.2006 |
| Citation | van de Graaf SF, et al. (2006) Direct Interaction with Rab11a Targets the Epithelial Ca2+ Channels TRPV5 and TRPV6 to the Plasma Membrane. Mol Cell Biol 26(1):303-12 |
| abstractText | TRPV5 and TRPV6 are the most Ca(2+)-selective members of the transient receptor potential (TRP) family of cation channels and play a pivotal role in the maintenance of Ca(2+) balance in the body. However, little is known about the mechanisms controlling the plasma membrane abundance of these channels to regulate epithelial Ca(2+) transport. In this study, we demonstrated the direct and specific interaction of GDP-bound Rab11a with TRPV5 and TRPV6. Rab11a colocalized with TRPV5 and TRPV6 in vesicular structures underlying the apical plasma membrane of Ca(2+)-transporting epithelial cells. This GTPase recognized a conserved stretch in the carboxyl terminus of TRPV5 that is essential for channel trafficking. Furthermore, coexpression of GDP-locked Rab11a with TRPV5 or TRPV6 resulted in significantly decreased Ca(2+) uptake, caused by diminished channel cell surface expression. Together, our data demonstrated the important role of Rab11a in the trafficking of TRPV5 and TRPV6. Rab11a exerts this function in a novel fashion, since it operates via direct cargo interaction while in the GDP-bound configuration. |
