| First Author | Hudson FN | Year | 2000 |
| Journal | Biochim Biophys Acta | Volume | 1492 |
| Issue | 2-3 | Pages | 447-51 |
| PubMed ID | 10899580 | Mgi Jnum | J:63622 |
| Mgi Id | MGI:1861302 | Doi | 10.1016/s0167-4781(00)00128-7 |
| Citation | Hudson FN, et al. (2000) Cloning and characterization of the proximal promoter region of the mouse glutamate-L-cysteine ligase regulatory subunit gene. Biochim Biophys Acta 1492(2-3):447-51 |
| abstractText | We describe upregulation of the mRNA for the mouse glutamate-cysteine ligase regulatory subunit gene (Glcl-r) in Hepa-1 cells treated with beta-napthoflavone (BNF) and tert-butylhydroquinone (tBHQ). A 2-kb fragment of the proximal promoter region of the gene was cloned and sequenced, and sequence analysis reveals a high degree of homology when compared to the human glutamate-cysteine ligase regulatory subunit gene promoter. Primer extension analysis indicates a major transcription start site 218 bp upstream of the translation start codon in a CpG-rich region, suggesting that transcription is Sp1 mediated. Reporter constructs containing nested deletion fragments of the Glcl-r promoter demonstrate that regulatory elements sufficient for basal and tBHQ-inducible expression lie between -273 and -787 bp relative to the translation start codon and that the distal promoter may contain negative regulatory elements. |
