| First Author | O E | Year | 2014 |
| Journal | Immunology | Volume | 142 |
| Issue | 4 | Pages | 624-35 |
| PubMed ID | 24645831 | Mgi Jnum | J:218574 |
| Mgi Id | MGI:5617928 | Doi | 10.1111/imm.12287 |
| Citation | O E, et al. (2014) Distinct B-cell populations contribute to vaccine antigen-specific antibody production in a transgenic mouse model. Immunology 142(4):624-35 |
| abstractText | The generation of memory B cells by vaccination plays a critical role in maintaining antigen-specific antibodies and producing antibody responses upon re-exposure to a pathogen. B-cell populations contributing to antibody production and protection by vaccination remain poorly defined. We used influenza virus-like particle (VLP) vaccine in a transgenic mouse model that would identify germinal centre-derived memory B cells with the expression of yellow fluorescent protein (YFP(+) cells). Immunization with influenza VLP vaccine did not induce significant increases in YFP(+) cells although vaccine antigen-specific antibodies in sera were found to confer protection against a lethal dose of influenza A virus (A/PR8). In addition, CD43(+) B220(-) populations with low YFP(+) cells mainly contributed to the production of vaccine antigen-specific IgG isotype-switched antibodies whereas CD43(-) B220(+) populations with high YFP(+) cells were able to produce vaccine antigen-specific IgM antibodies. Challenge infection of immunized transgenic mice with live influenza A virus resulted in significant increases in YFP(+) cells in the B220(-) populations of spleen and bone marrow cells. These results suggest that CD43(+) B220(-) B cells generated by vaccination are important for producing influenza vaccine antigen-specific antibodies and conferring protection. |
