| First Author | Kusche-Gullberg M | Year | 1998 |
| Journal | J Biol Chem | Volume | 273 |
| Issue | 19 | Pages | 11902-7 |
| PubMed ID | 9565617 | Mgi Jnum | J:47442 |
| Mgi Id | MGI:1203445 | Doi | 10.1074/jbc.273.19.11902 |
| Citation | Kusche-Gullberg M, et al. (1998) Identification and expression in mouse of two heparan sulfate glucosaminyl N-deacetylase/N-sulfotransferase genes. J Biol Chem 273(19):11902-7 |
| abstractText | The biosynthesis of heparan sulfate/heparin is a complex process that requires the coordinate action of a number of different enzymes. In close connection with polymerization of the polysaccharide chain, the modification reactions are initiated by N-deacetylation followed by N- sulfation of N-acetylglucosamine units. These two reactions are carried out by a single protein. Proteins with such dual activities were first purified and cloned from rat liver and mouse mastocytoma. The mouse mastocytoma enzyme is encoded by an approximately 4-kilobase (kb) mRNA, whereas the rat liver transcript contains approximately 8 kb. In the present study, the primary structure of the enzyme encoded by the mouse 8-kb transcript is described. It is demonstrated that both the 4-and 8- kb transcripts have a wide tissue distribution and that they are encoded by separate genes. Characterization of the gene encoding the 4- kb transcript demonstrates that it spans a region of about 8 kb and that it contains at least 14 exons. The similarity of this gene and the previously characterized human gene for the 8-kb transcript is discussed. |
