| First Author | Mann VM | Year | 1992 |
| Journal | Comp Biochem Physiol B | Volume | 102 |
| Issue | 3 | Pages | 551-9 |
| PubMed ID | 1499292 | Mgi Jnum | J:2198 |
| Mgi Id | MGI:50722 | Doi | 10.1016/0305-0491(92)90047-u |
| Citation | Mann VM, et al. (1992) Subcellular fractionation evidence for a putative peroxisome-mitochondrion attachment in the liver of normal and genetically obese (ob/ob and db/db) mice. Comp Biochem Physiol [B] 102(3):551-9 |
| abstractText | 1. Liver post-nuclear supernatants (PNS) from several mouse strains were fractionated by zonal centrifugation and fractions analysed by marker-enzyme estimations+electron microscopy. 2. Rate-dependent banding of PNS yielded peroxisome-enriched (PER) and mitochondrion-enriched (MER) regions. 3. Density-dependent banding of PER yielded peroxisomes (approximately 1.22 g/ml) well separated from mitochondria (approximately 1.8 g/ml). 4. Density-dependent banding of MER yielded peroxisomes that co-distributed with mitochondria and electron microscopy revealed close proximity of the two organelles. 5. Experiments demonstrated that co-distribution was not due to weak binding of proteins or to agglutination of organelles. 6. The results indicate in vivo attachment of some mitochondria and peroxisomes. |
