Primary Identifier | MGI:2149765 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ephb2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A PGK-neomycin resistance cassette replaced 1.4kb of sequence including the exon that encodes amino acids 29-50, and introduced a frame shift downstream of the insertion. In an immune-complex in vitro tyrosine kinase assay, antiserum raised against the C-terminal of the protein did not detect wild-type protein in lysates of homozygous mutant E10.5 embryos. |