| First Author | Isono K | Year | 2013 |
| Journal | Dev Cell | Volume | 26 |
| Issue | 6 | Pages | 565-77 |
| PubMed ID | 24091011 | Mgi Jnum | J:204987 |
| Mgi Id | MGI:5543846 | Doi | 10.1016/j.devcel.2013.08.016 |
| Citation | Isono K, et al. (2013) SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Dev Cell 26(6):565-77 |
| abstractText | The Polycomb-group (PcG) repressive complex-1 (PRC1) forms microscopically visible clusters in nuclei; however, the impact of this cluster formation on transcriptional regulation and the underlying mechanisms that regulate this process remain obscure. Here, we report that the sterile alpha motif (SAM) domain of a PRC1 core component Phc2 plays an essential role for PRC1 clustering through head-to-tail macromolecular polymerization, which is associated with stable target binding of PRC1/PRC2 and robust gene silencing activity. We propose a role for SAM domain polymerization in this repression by two distinct mechanisms: first, through capturing and/or retaining PRC1 at the PcG targets, and second, by strengthening the interactions between PRC1 and PRC2 to stabilize transcriptional repression. Our findings reveal a regulatory mechanism mediated by SAM domain polymerization for PcG-mediated repression of developmental loci that enables a robust yet reversible gene repression program during development. |
