Primary Identifier | MGI:5588003 | Allele Type | Targeted |
Attribute String | Conditional ready, Reporter | Gene | Dlg4 |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6J)F1 |
Is Recombinase | false | Is Wild Type | false |
description | ES cell line = G1 (129S6/SvEvTac x C57BL/6J)F1. |
molecularNote | The last two exons of the gene (exons 19 and 20), including the 3' UTR, were duplicated and inserted downstream of the polyadenylation signal and a neomycin selection cassette flanked by FRT sites. Two loxP sites were inserted to flank the native exons 19 and 20 as well as a neomycin stop cassette. The sequence for mVenus was fused in frame to the end of the coding sequence in exon 20. The FRT-flanked neomycin cassette was excised through crosses with a germline flp strain. |