| First Author | Li H | Year | 2008 |
| Journal | Nat Cell Biol | Volume | 10 |
| Issue | 11 | Pages | 1301-8 |
| PubMed ID | 18836438 | Mgi Jnum | J:145632 |
| Mgi Id | MGI:3835699 | Doi | 10.1038/ncb1788 |
| Citation | Li H, et al. (2008) Actin-driven chromosomal motility leads to symmetry breaking in mammalian meiotic oocytes. Nat Cell Biol 10(11):1301-8 |
| abstractText | Movement of meiosis I (MI) chromosomes from the oocyte centre to a subcortical location is the first step in the establishment of cortical polarity. This is required for two consecutive rounds of asymmetric meiotic cell divisions, which generate a mature egg and two polar bodies. Here we use live-cell imaging and genetic and pharmacological manipulations to determine the force-generating mechanism underlying this chromosome movement. Chromosomes were observed to move toward the cortex in a pulsatile manner along a meandering path. This movement is not propelled by myosin-II-driven cortical flow but is associated with a cloud of dynamic actin filaments trailing behind the chromosomes/spindle. Formation of these filaments depends on the actin nucleation activity of Fmn2, a formin-family protein that concentrates around chromosomes through its amino-terminal region. Symmetry breaking of the actin cloud relative to chromosomes, and net chromosome translocation toward the cortex require actin turnover. |
