Primary Identifier | MGI:2183220 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Fut2 |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
description | The embryonic stem cell lines Pat5 and R1 were used for gene targeting. |
molecularNote | Most of the single coding exon of the gene was replaced with a lacZ-PGK-neo cassette via homologous recombination resulting in deletion of the catalytic domain. Homozygous mutant animals were identified by Southern blot and PCR genotype analysis. |