| First Author | Satoh T | Year | 2009 |
| Journal | Biochem Biophys Res Commun | Volume | 389 |
| Issue | 4 | Pages | 673-7 |
| PubMed ID | 19766595 | Mgi Jnum | J:155005 |
| Mgi Id | MGI:4412137 | Doi | 10.1016/j.bbrc.2009.09.056 |
| Citation | Satoh T, et al. (2009) Isolation of a novel leptin receptor gene promoter preferentially functioning in neuronal cells. Biochem Biophys Res Commun 389(4):673-7 |
| abstractText | Leptin exerts its metabolic effects by binding to the leptin receptor (Ob-R). In humans, the promoter of the Ob-R gene-related protein (Ob-RGRP) gene and the B219/Ob-R promoter have been speculated to spatially and temporarily regulate Ob-R gene transcription; however, the promoter function of the Ob-R gene has not been directly analyzed. Using 5' rapid amplification of the cDNA end, we isolated novel ob-r transcripts starting from the 3' portions of the B219/ob-r 5'-untranslated region in the adult mouse brain. The proximal promoter containing these start sites showed robust activity in neuron-derived, but not non-neuronal cell lines. The promoter activity was comparable with that of the ob-rgrp promoter in a neuronal cell, but significantly weaker in non-neuronal cells. Deletion analyses identifying two enhancer elements were located in this promoter. The identification of a novel ob-r gene promoter might provide a useful tool to study neuron specific expression and hormonal regulation of the ob-r gene. |
