| First Author | Koh CP | Year | 2023 |
| Journal | Gene | Volume | 851 |
| Pages | 147049 | PubMed ID | 36384171 |
| Mgi Jnum | J:331396 | Mgi Id | MGI:7387939 |
| Doi | 10.1016/j.gene.2022.147049 | Citation | Koh CP, et al. (2023) Highly efficient Runx1 enhancer eR1-mediated genetic engineering for fetal, child and adult hematopoietic stem cells. Gene 851:147049 |
| abstractText | A cis-regulatory genetic element which targets gene expression to stem cells, termed stem cell enhancer, serves as a molecular handle for stem cell-specific genetic engineering. Here we show the generation and characterization of a tamoxifen-inducible CreER(T2) transgenic (Tg) mouse employing previously identified hematopoietic stem cell (HSC) enhancer for Runx1, eR1 (+24 m). Kinetic analysis of labeled cells after tamoxifen injection and transplantation assays revealed that eR1-driven CreER(T2) activity marks dormant adult HSCs which slowly but steadily contribute to unperturbed hematopoiesis. Fetal and child HSCs that are uniformly or intermediately active were also efficiently targeted. Notably, a gene ablation at distinct developmental stages, enabled by this system, resulted in different phenotypes. Similarly, an oncogenic Kras induction at distinct ages caused different spectrums of malignant diseases. These results demonstrate that the eR1-CreER(T2) Tg mouse serves as a powerful resource for the analyses of both normal and malignant HSCs at all developmental stages. |
