| First Author | Bi J | Year | 2019 |
| Journal | Cytokine | Volume | 114 |
| Pages | 135-142 | PubMed ID | 30467097 |
| Mgi Jnum | J:295593 | Mgi Id | MGI:6454091 |
| Doi | 10.1016/j.cyto.2018.11.011 | Citation | Bi J, et al. (2019) Inflammasome and cytokine expression profiling in experimental periodontitis in the integrin beta6 null mouse. Cytokine 114:135-142 |
| abstractText | Epithelial alphavbeta6 integrin participates in immune surveillance in many organs, including the gastrointestinal track. Expression of alphavbeta6 integrin is reduced in the junctional epithelium of the gingiva in periodontal diseases, and mutations in the ITGB6 gene are associated with these diseases in humans and mice. The aim of this study was to unravel potential differences in the inflammatory responses in the periodontal tissues of FVB wild-type (WT) and beta6 integrin-null (Itgb6(-/-)) mice, using a ligature-induced periodontitis model and assessing inflammation, bone loss and expression profiles of 34 genes associated with periodontal disease. Using micro-CT and histology, we demonstrated more advanced inflammation and bone loss in the control and ligatured Itgb6(-/-) mice compared to the WT animals. Neutrophil and macrophage marker genes were significantly upregulated by ligation in both WT and Itgb6(-/-) mice while the expression of T-cell and B-cell markers was downregulated, suggesting acute-type of inflammation. Expression of inflammasome NLRP3-related genes Nlpr3 and Il1b was also significantly increased in both groups. However, the expression of Il18 was significantly lower in non-ligatured Itgb6(-/-) mice than in the WT mice and was further downregulated in both groups by the ligatures. IL-18 mediates many effects of the AIM2 inflammasome, including regulation of the microbiome. Interestingly, expression of Aim2 was significantly lower in both control and ligatured Itgb6(-/-) mice than in WT animals. Overall, ligature-induced periodontitis was associated with increased expression of pro-inflammatory cytokines, chemokines and osteoclastogenic regulatory molecules. Another significant difference between the Itgb6(-/-) and WT mice was that mRNA expression of the anti-inflammatory cytokine IL-10 was increased in ligatured WT mice but reduced in the Itgb6(-/-) mice. In conclusion, alphavbeta6 integrin in junctional epithelium of the gingiva appears to positively regulate the expression of the AIM2 inflammasome and anti-inflammatory IL-10, thus providing protection against periodontal inflammation. |
