| First Author | Schulz M | Year | 1995 |
| Journal | Eur J Immunol | Volume | 25 |
| Issue | 2 | Pages | 474-80 |
| PubMed ID | 7533086 | Mgi Jnum | J:23268 |
| Mgi Id | MGI:71034 | Doi | 10.1002/eji.1830250225 |
| Citation | Shulz M, et al. (1995) Acute rejection of vascular heart allografts by perforin- deficient mice. Eur J Immunol 25(2):474-480 |
| abstractText | To study the role of perforin in cell-mediated graft rejection, vascularized hearts were grafted to perforin- deficient C57BL/6 and control C57BL/6 recipient mice. Fully allogeneic heart grafts (BALB/c) were acutely rejected by both recipients within 6 days. Peritoneal exudate lymphocytes from control mice but not from perforin-deficient mice exhibit a strong alloreactive cytotoxic activity in vitro. Histological analysis of the rejected tissues demonstrated extensive mononuclear cell infiltrates in both recipients. Flow cytometry analysis and immunohistology of graft-infiltrating cells showed similar proportions of lymphocyte subsets (CD8 much greater than CD4). Collectively, these data indicate that perforin is not essential in the cell-mediated acute rejection of a fully mismatched heart allograft. However, perforin-dependent effector mechanisms appeared to be limiting in the T cell-mediated rejection of heart allografts differing only at a single major histocompatibility complex class I antigen (bm1), because these grafts survived longer (mean 87.8 days) in perforin- deficient than in control mice (mean 31.5 days). |
