| First Author | Lee MW | Year | 2001 |
| Journal | J Biomed Sci | Volume | 8 |
| Issue | 6 | Pages | 504-11 |
| PubMed ID | 11702014 | Mgi Jnum | J:283453 |
| Mgi Id | MGI:6388475 | Doi | 10.1007/bf02256613 |
| Citation | Lee MW, et al. (2001) Restricted expression of LUZP in neural lineage cells: a study in embryonic stem cells. J Biomed Sci 8(6):504-11 |
| abstractText | A novel protein LUZP with 3 leucine zipper motifs at its amino terminus is predominantly expressed in the adult brain. A modified gene targeting approach was employed in an attempt to establish in vitro and in vivo models in which Luzp is knock-out (KO) for phenotype assessment and a reporter gene lacZ is knock-in (KI) for tracing its expression. We report in this study the molecular cloning of the Luzp gene, its targeting vector construction and Luzp-KO/lacZ-KI embryonic stem (ES) clone selection. Since LUZP is also expressed in ES cells, the possibility of embryonic lethality cannot be excluded when attempting to establish Luzp-null mutant mice. We have therefore examined the development of homozygous Luzp-KO/lacZ-KI clones in nude mice. Tissue types derived from all three embryonic germ layers were observed in teratomas developed in nude mice. In situ X-gal staining further revealed restricted expression of LUZP in neural lineage cells. |
