| First Author | Tighe H | Year | 2000 |
| Journal | Eur J Immunol | Volume | 30 |
| Issue | 7 | Pages | 1939-47 |
| PubMed ID | 10940883 | Mgi Jnum | J:63508 |
| Mgi Id | MGI:1861081 | Doi | 10.1002/1521-4141(200007)30:7<1939::AID-IMMU1939>3.0.CO;2-# |
| Citation | Tighe H, et al. (2000) Conjugation of protein to immunostimulatory DNA results in a rapid, long-lasting and potent induction of cell-mediated and humoral immunity. Eur J Immunol 30(7):1939-47 |
| abstractText | Immunostimulatory DNA sequences (ISS) are a potent Th1 adjuvant. We hypothesized that conjugation of ISS to protein antigens would strongly enhance their immunogenicity because both antigen and adjuvant (ISS) would be delivered to the same locale/antigen-presenting cell. To test this hypothesis, we conjugated a 22-mer immunostimulatory oligodeoxynucleotide (ISS-ODN) to two test antigens of differing intrinsic immunogenicity, namely Escherichia coli beta-galactosidase and the HIV-1 envelope glycoprotein gp120. We show that the antigen-ISS conjugates rapidly induce Th1 cells secreting high levels of IFN-gamma, strong CTL activity, and high titer IgG2a and HIV-neutralizing antibodies, exceeding gene and protein vaccination alone or immunization with mixtures of antigen and ISS-ODN. The data suggest that this procedure generates a novel and unique vaccine that rapidly triggers strong humoral and cell-mediated immunity. |
