Primary Identifier | MGI:2182148 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ncan |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin selection cassette was inserted into exon 3, which encodes the immunoglobulin-like domain. Transcript was detected in homozygous mutant mice via Norhtern blot analysis. RT-PCR revealed that the detected signal was that of an aberrant transcript from which exon 3 had been excised. Western blot analysis showed an absence of protein. |