| First Author | Autio A | Year | 2022 |
| Journal | PLoS One | Volume | 17 |
| Issue | 4 | Pages | e0266566 |
| PubMed ID | 35413056 | Mgi Jnum | J:324022 |
| Mgi Id | MGI:7262370 | Doi | 10.1371/journal.pone.0266566 |
| Citation | Autio A, et al. (2022) SIRPalpha - CD47 axis regulates dendritic cell-T cell interactions and TCR activation during T cell priming in spleen. PLoS One 17(4):e0266566 |
| abstractText | The SIRPalpha-CD47 axis plays an important role in T cell recruitment to sites of immune reaction and inflammation but its role in T cell antigen priming is incompletely understood. Employing OTII TCR transgenic mice bred to Cd47-/- (Cd47KO) or SKI mice, a knock-in transgenic animal expressing non-signaling cytoplasmic-truncated SIRPalpha, we investigated how the SIRPalpha-CD47 axis contributes to antigen priming. Here we show that adoptive transfer of Cd47KO or SKI Ova-specific CD4+ T cells (OTII) into Cd47KO and SKI recipients, followed by Ova immunization, elicited reduced T cell division and proliferation indices, increased apoptosis, and reduced expansion compared to transfer into WT mice. We confirmed prior reports that splenic T cell zone, CD4+ conventional dendritic cells (cDCs) and CD4+ T cell numbers were reduced in Cd47KO and SKI mice. We report that in vitro derived DCs from Cd47KO and SKI mice exhibited impaired migration in vivo and exhibited reduced CD11c+ DC proximity to OTII T cells in T cell zones after Ag immunization, which correlates with reduced TCR activation in transferred OTII T cells. These findings suggest that reduced numbers of CD4+ cDCs and their impaired migration contributes to reduced T cell-DC proximity in splenic T cell zone and reduced T cell TCR activation, cell division and proliferation, and indirectly increased T cell apoptosis. |
