| First Author | Parr RL | Year | 1995 |
| Journal | J Virol | Volume | 69 |
| Issue | 8 | Pages | 5033-8 |
| PubMed ID | 7609073 | Mgi Jnum | J:26864 |
| Mgi Id | MGI:74291 | Doi | 10.1128/jvi.69.8.5033-5038.1995 |
| Citation | Parr RL, et al. (1995) Association of mouse fibrinogen-like protein with murine hepatitis virus-induced prothrombinase activity. J Virol 69(8):5033-8 |
| abstractText | Previously, we demonstrated induction of a unique macrophage prothrombinase during infection of BALB/cJ mice by mouse hepatitis virus strain 3 (MHV-3). By immunologic screening, a clone representing this prothrombinase was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a fibrinogen-like protein. Six additional clones were isolated, and one clone, p11-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constitutively express musfiblp but, when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrated when musfiblp was expressed from p11-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase. |
