| First Author | Takahashi H | Year | 2015 |
| Journal | Nat Commun | Volume | 6 |
| Pages | 5941 | PubMed ID | 25575120 |
| Mgi Jnum | J:264970 | Mgi Id | MGI:6199010 |
| Doi | 10.1038/ncomms6941 | Citation | Takahashi H, et al. (2015) MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex. Nat Commun 6:5941 |
| abstractText | Regulation of transcription elongation by RNA polymerase II (Pol II) is a key regulatory step in gene transcription. Recently, the little elongation complex (LEC)-which contains the transcription elongation factor ELL/EAF-was found to be required for the transcription of Pol II-dependent small nuclear RNA (snRNA) genes. Here we show that the human Mediator subunit MED26 plays a role in the recruitment of LEC to a subset of snRNA genes through direct interaction of EAF and the N-terminal domain (NTD) of MED26. Loss of MED26 in cells decreases the occupancy of LEC at a subset of snRNA genes and results in a reduction in their transcription. Our results suggest that the MED26-NTD functions as a molecular switch in the exchange of TBP-associated factor 7 (TAF7) for LEC to facilitate the transition from initiation to elongation during transcription of a subset of snRNA genes. |
