Primary Identifier | MGI:4421180 | Allele Type | Targeted |
Attribute String | Not Specified | Gene | Bmf |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed neo cassette was inserted within intron 3, and specific nucleotide mutations in exon 3 were introduced by homologous recombination. Mutation resulted in the JNK protein kinase phosphorylation site (Ser-74) in exon 3 to be substituted with an Ala (S74A). The neo cassette was then excised with Cre mediated recombination leaving a single loxP site in intron 3. |